YY super sperm lead to all male triploids and tetraploids

• DOI: 10.1186/s12863-015-0230-z
• Publication Date: 2015-06-25T04:07:28Z
• Authors: Rong Zhou; Jun Xiao; Qinbo Qin; Bin Zhu; Rurong Zhao; Chun Zhang; Min Tao; Kaikun Luo; Jing Wang; Liangyue Peng; Shaojun Liu
• Keywords: Crucian carp; Common carp

Androgenesis is a unique and rarely encountered reproductive mode in which the offspring only inherit paternal nuclear genome, resulting relatively few viable individuals. In this study, super male (YY) crucian carp was obtained by androgenesis with diploid sperm of autotetraploid (4n = 200). Flow cytometry assay confirmed fish diploid. The scanning electron microscopy flow analysis results revealed that YY produced unreduced sperm. To prove special characteristic homozygosity carp, three rounds hybridization experiments were performed. First, self-crossing between female androgenic progenies generated all tetraploids. Then, red (2n 100) 200) triploids tetraploids, respectively. This first time reported producing homozygous fish, derived from distant hybridization. These will not help explaining sex determination mechanism teleost but also play significant role genetic breeding aquaculture.