Defining the gene repertoire and spatiotemporal expression profiles of adhesion G protein-coupled receptors in zebrafish

• DOI: 10.1186/s12864-015-1296-8
• Publication Date: 2015-02-07T14:57:16Z
• Authors: Breanne L Harty; Arunkumar Krishnan; Nicholas E Sanchez; Helgi B Schiöth; Kelly R Monk
• Keywords: Model Organism; Developmental Biology

Adhesion G protein-coupled receptors (aGPCRs) are the second largest of five GPCR families and essential for a wide variety physiological processes. Zebrafish have proven to be very effective model studying biological functions aGPCRs in both developmental adult contexts. However, aGPCR repertoires not been defined any fish species, nor expression profiles tissues known. Additionally, family never extensively characterized over time-course species. Here, we report that there at least 59 zebrafish represent homologs 24 33 found humans; compared humans, lack clear GPR110, GPR111, GPR114, GPR115, GPR116, EMR1, EMR2, EMR3, EMR4. We find several multiple paralogs, line with teleost-specific genome duplication. Phylogenetic analysis suggests most cluster closely their mammalian homologs, exception three zebrafish-specific expansion events Groups II, VI, VIII. Using quantitative real-time PCR, 12 time points 10 representing every major organ system. Importantly, similar those previously reported mouse, rat, human, underscoring evolutionary conservation this family, therefore utility biology. Our results support notion potentially useful study biology from functional perspective. The repertoire, classification, nomenclature, together during development tissues, provides crucial foundation elucidating pursuing as therapeutic targets.