Microarray: a global analysis of biomineralization-related gene expression profiles during larval development in the pearl oyster, Pinctada fucata

• DOI: 10.1186/s12864-015-1524-2
• Publication Date: 2015-04-18T06:36:05Z
• Authors: Jun Liu; Dong Yang; Shiting Liu; Shiguo Li; Guangrui Xu; Guilan Zheng; Liping Xie; Rongqing Zhang
• Keywords: Pinctada fucata; Echinoderm

The molluscan Pinctada fucata is an important pearl-culturing organism to study biomineralization mechanisms. Several biomineralization-related genes play roles regulating shell formation, but most previous work has focused only on their functions in adult oysters. Few studies have investigated during larval development, when the initially constructed and formed until juvenile stage dissoconch shells. Here, we report, for first time, a global gene analysis development of P. based microarray reveal relationships between formation process.Based mantle transcriptome, 58,940 probes (60 nt), representing 58,623 transcripts, were synthesized. expression profiles fertilized egg, trochophore, D-shaped, umbonal larvae, as well juveniles analyzed by performance. patterns changed corresponding regulatory function formation. Matrix proteins chitin synthase PFMG2 highly expressed at D-shaped stage, whereas PFMG6, PFMG8 PfN23 significantly up-regulated indicating different either periostracum, Prodissoconch I or II However, majority matrix high levels shells comprised both aragonitic nacreous layer calcitic prismatic adults. We also identified five new that juveniles. These particularly coded secreted with tandem-arranged repeat units, proteins. RNAi knockdown resulted disrupted layers, potential formation.Our results add perspective propose mechanism how regulate process. increase knowledge about highlight aspects