Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus

• DOI: 10.1186/s12864-015-1599-9
• Publication Date: 2015-05-15T10:12:19Z
• Authors: Eike J Steinig; Patiyan Andersson; Simon R Harris; Derek S Sarovich; Anand Manoharan; Paul Coupland; Matthew TG Holden; Julian Parkhill; Stephen D Bentley; D Ashley Robinson; Steven YC Tong
• Keywords: Lineage (genetic); Panton–Valentine leukocidin; Multilocus sequence typing

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-associated infection, but there growing awareness the emergence multidrug-resistant lineages in community settings around world. One such lineage ST772-MRSA-V, which has disseminated globally and increasingly prevalent India. Here, we present complete genome sequence DAR4145, strain ST772-MRSA-V from India, investigate its genomic characteristics regards to antibiotic resistance virulence factors.Sequencing using single-molecule real-time technology resulted assembly single continuous chromosomal sequence, was error-corrected, annotated compared nine draft assemblies Australia, Malaysia We discovered numerous redundant genes associated with mobile genetic elements (MGEs) known core mutations that explain highly resistant phenotype DAR4145. Staphylococcal toxins superantigens, including leukotoxin Panton-Valentinin Leukocidin, were predominantly islands phage φ-IND772PVL. Some these factors variably other strains lineage.The presented here emphasize contribution MGEs virulent community-associated MRSA. Antibiotic further augmented by redundancy genes. The DAR4145 provides valuable resource for future investigations into global dissemination phylogeography ST772-MRSA-V.