Major differential gene regulation in Coxiella burnetii between in vivo and in vitro cultivation models

• DOI: 10.1186/s12864-015-2143-7
• Publication Date: 2015-11-16T11:37:14Z
• Authors: Runa Kuley; Ruth Bossers-deVries; Hilde E. Smith; Mari A. Smits; Hendrik I. J. Roest; Alex Bossers
• Keywords: Coxiella burnetii; Q fever; Intracellular parasite

Coxiella burnetii is the causative agent of zoonotic disease Q fever. As it an intracellular pathogen, infection by C. requires adaptation to its eukaryotic host and environment. The recently developed cell-free medium also allows bacteria propagate without cells, maintaining potential. different hosts or extracellular environments has been assumed involve genome-wide modulation gene expression. However, little currently known about these events which are critical for understanding survival burnetii. We studied genome–wide transcriptional patterns in vivo (mice spleen) cell vitro culture models examine metabolic pathways virulence associated expression that required colonize persist environments. Within each model, profiles Dutch outbreak strain (602) NM reference strains were largely similar. In contrast, gene-expression was strongly influenced cultivation method, indicating bacterium available components. Genome–wide from more similar those seen conditions, while distant vivo. Under significant alterations genes involved metabolism identified. observed under conditions predominantly uses glucose as a carbon source (mostly biosynthetic processes) fatty acids energy generation. experienced nutrient limitation anaerobiosis major stressors, phosphate identified important signal growth inside cells. Finally, environment significantly induced several genes, including implicated LPS synthesis, colonization, component DNA repair mechanisms. Our study shows burnetii, with relative small genome, only subset core functions survive but induction full repertoire successful pathogenesis thriving harsh