Comparative genome analysis of Weissella ceti, an emerging pathogen of farm-raised rainbow trout

• DOI: 10.1186/s12864-015-2324-4
• Publication Date: 2015-12-22T13:11:30Z
• Authors: Henrique C. P. Fi...; Siomar C. Soares; Felipe L. Pereira; Fernanda A. Dorella; Alex F. Carvalho; Júnia P. Teixeira; Vasco A. C. Azevedo; Carlos A. G. Leal
• Keywords: Weissella; Synteny; Minion; Comparative Genomics

The genus Weissella belongs to the lactic acid bacteria and includes 18 currently identified species, predominantly isolated from fermented food but rarely cases of bacteremia in animals. Recently, a new designated ceti, has been correlated with hemorrhagic illness farm-raised rainbow trout China, Brazil, USA, high transmission mortality rates during outbreaks. Although W. ceti is an important emerging veterinary pathogen, little known about its genomic features or virulence mechanisms. To better understand these characterize we have previously sequenced genomes strains WS08, WS74, WS105, different farms Brazil displaying pulsed-field gel electrophoresis patterns. Here, present comparative analysis three along NC36 USA those other species. Phylogenomic orthology-based analyses both showed high-similarity genetic structure strains. This corroborated by highly syntenic order their genes neutral evolution inferred Tajima's D. A whole-genome multilocus sequence typing distinguished WS08 WS74 WS105. We predicted 10 putative islands (GEI), among which PAIs 3a 3b are phage sequences that occur only WS105 respectively, whereas PAI 1 species specific. several putatively involved basic processes bacterial physiology pathogenesis, including survival aquatic environment, adherence host, spread inside resistance immune-system-mediated stresses, antibiotic resistance. These data provide insights molecular epidemiology host adaptation for this pathogen aquaculture.