Genome-wide analysis of tomato NF-Y factors and their role in fruit ripening

• DOI: 10.1186/s12864-015-2334-2
• Publication Date: 2016-01-07T22:56:50Z
• Authors: Shan Li; Ka Li; Zheng Ju; Dongyan Cao; Daqi Fu; Hongliang Zhu; Benzhong Zhu; Yunbo Luo
• Keywords: MYB

Fruit ripening is a complex developmental process that depends on coordinated regulation of numerous genes, including ripening-related transcription factors (TFs), fruit-related microRNAs, DNA methylation and chromatin remodeling. It known various TFs, such as MADS-domain, MYB, AP2/ERF SBP/SPL family proteins play key roles in modulating ripening. However, little attention has been given to members the large NF-Y TF this regard, although genes are have important functions regulating plant growth, development, abiotic or biotic stress responses. In study, evolutionary relationship between Arabidopsis thaliana tomato (Solanum lycopersicum) was examined predict similarities function. Furthermore, through gene expression analysis, 13 were identified candidate regulators fruit Functional studies involving suppression using virus induced silencing (VIGS) indicated five two NF-YB subgroup (Solyc06g069310, Solyc07g065500) three NF-YA (Solyc01g087240, Solyc08g062210, Solyc11g065700), influence addition, subcellular localization analyses fused green fluorescent protein (GFP) reporter showed accumulated nucleus, while observed both nucleus cytoplasm. we by analyzing genome sequence bioinformatics approaches, characterized their chromosomal distribution, structures, phylogenetic patterns. We also biological via VIGS analyses. The results This information provides platform for further investigation functions.