RNA-seq analysis of virR and revR mutants of Clostridium perfringens

• DOI: 10.1186/s12864-016-2706-2
• Publication Date: 2016-05-24T00:20:40Z
• Authors: Lee-Yean Low; Paul F. Harrison; Ya-Hsun Lin; John D. Boyce; Julian I. Rood; Jackie K. Cheung
• Keywords: Clostridium perfringens; Gas gangrene

Clostridium perfringens causes toxin-mediated diseases, including gas gangrene (clostridial myonecrosis) and food poisoning in humans. The production of the toxins implicated gangrene, α-toxin perfringolysin O, is regulated by VirSR two-component regulatory system. In addition, RevR, an orphan response regulator, has been shown to affect virulence mouse myonecrosis model. RevR positively regulates expression genes that encode hydrolytic enzymes, hyaluronidases sialidases. To further characterize networks, comparative transcriptomic analysis was carried out with strand-specific RNA-seq on C. strain JIR325 its isogenic virR revR mutants. Using edgeR package, 206 mutant 67 were found be differentially expressed. Comparative revealed VirR acts as a global negative whilst positive regulator. Therefore, about 95 % expressed up-regulated mutant, whereas 81 down-regulated mutant. Importantly, we identified 23 both 18 these genes, which included sporulation-specific spoIVA, sigG sigF negatively VirR, respectively. Furthermore, mapped reads visualized depth coverage plots showed there 93 previously unannotated transcripts intergenic regions. These potentially small RNA molecules. conclusion, using analysis, this study chromosomal pCP13 native plasmid-encoded antisense transcripts, within regions are controlled or systems.