High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying

• DOI: 10.1186/s12864-017-3923-z
• Publication Date: 2017-07-20T07:51:02Z
• Authors: Jin-Ling Feng; Zhi-Jian Yang; Shi-Pin Chen; Yousry A. El-Kassaby; Hui Chen
• Keywords: Camellia oleifera; Camellia; Fatty Acid Metabolism; KEGG; Secondary metabolism

Camellia species are ancient oilseed plants with a history of cultivation over two thousand years. Prior to oil extraction, natural seed drying is often practiced, process affecting fatty acid quality and quantity. MicroRNAs (miRNA) lipid metabolism associated camellia unexplored. To obtain insight into the function miRNAs in during drying, Illumina sequencing C. oleifera meiocarpa small-RNA was conducted.A total 274 candidate were identified 3733 target unigenes annotated by performing BLASTX. Through integrated GO KEGG annotation, 23 miRNA regulating 131 genes as metabolism, biosynthesis, accumulation catabolism. We observed one, two, four which specially expressed Meiocarpa, oleifera, collectively, respectively. At 30% moisture contents, oleifer produced nine eight significant differentially miRNAs, respectively, high synthesis activities. Across species, 12 at 50% content.Sequencing revealed presence permitted comparative profiles between Meiocarpa oleifera. Furthermore, this study successfully best environment quantity its maximum.