Proteomics informed by transcriptomics for characterising differential cellular susceptibility to Nelson Bay orthoreovirus infection

• DOI: 10.1186/s12864-017-3994-x
• Publication Date: 2017-08-14T12:06:32Z
• Authors: Lawrence Mok; James W. Wynne; Mary Tachedjian; Brian Shiell; Kris Ford; David A. Matthews; Antony Bacic; Wojtek P. Michalski
• Keywords: Proteome; Permissiveness; Cytopathic effect; Syncytium; Cell type

Nelson Bay orthoreovirus (NBV) is a fusogenic bat borne virus with an unknown zoonotic potential. Previous studies have shown that NBV can infect and replicate in wide variety of cell types derived from their natural host (bat), as well human, mouse monkey. Within permissive cells, induced significant cytopathic effects characterised by cell-cell fusion syncytia formation. To understand the molecular events underpin infection we examined transcriptome proteome response two types, (PaKiT03) (L929), to characterise differential cellular susceptibility NBV. Despite differences replication L929 PaKiT03 was remarkably similar these cells. At both level, dominated IFN production signalling pathways. The majority proteins up-regulated cells were also at mRNA (gene) included many important stimulated genes. Further functional experimentation demonstrated stimulating prior infection, significantly reduced Moreover, inhibiting (through specific siRNAs) increased In line seen observed down-regulation genes involved junctions, which may be related This study provides new multi-dimensional insights into mammalian infection. We show activity capable reducing replication, although it unlikely this solely responsible for described here will prove valuable identifying potential therapeutic targets against orthoreovirus.