Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum

• DOI: 10.1186/s12864-017-4398-7
• Publication Date: 2018-01-04T12:33:02Z
• Authors: Yingying Wang; Shouli Feng; Sai Li; Dong Tang; Yu Chen; Yu Chen; Baoliang Zhou
• Keywords: Introgression

We previously reported the development of a set Gossypium hirsutum-G. australe alien chromosome addition lines. Naturally, however, G. exchanges were very limited, impeding stable transference useful genes from (G2G2 genome) into most cultivated cotton, hirsutum (AADD). In present report, pollen pentaploid (2n = AADDG2) was irradiated with seven different doses ranging 10 to 40 Grays and used pollinate emasculated flowers over three consecutive years. Irradiation greatly increased genetic recombination rates chromosomes total 107 introgression individuals in 192 GISH-negative (with no GISH signal on chromosome) survived individuals, 11 translocation (containing 12 events) 67 obtained 70 GISH-positive signal(s) chromosome(s)) which are invaluable for mining desirable australe. Multicolor genomic situ hybridization results showed that there types translocation, whole arm large segment small all translocations occurred between G2-genome A-subgenome hirsutum. also found higher induced much variation but lowered seed viability seedling survivability. has been successfully employed induce introgressions promote wild species. addition, by balancing those set, germination, M1 generation, we conclude dosage 20 is suitable. The established methodology may guide utilization tertiary gene pool species such as cotton breeding future.