The genome of Rhizophagus clarus HR1 reveals a common genetic basis for auxotrophy among arbuscular mycorrhizal fungi

• DOI: 10.1186/s12864-018-4853-0
• Publication Date: 2018-06-18T11:44:49Z
• Authors: Yuuki Kobayashi; Taro Maeda; Katsushi Yamaguchi; Hiromu Kameoka; Sachiko Tanaka; Tatsuhiro Ezawa; Shuji Shigenobu; Masayoshi Kawaguchi
• Keywords: Rhizophagus irregularis; Metabolic pathway; Obligate; KEGG

Mycorrhizal symbiosis is one of the most fundamental types mutualistic plant-microbe interaction. Among many classes mycorrhizae, arbuscular mycorrhizae have general symbiotic style and longest history. However, genomes mycorrhizal (AM) fungi are not well characterized due to difficulties in cultivation genetic analysis. In this study, we sequenced genome AM fungus Rhizophagus clarus HR1, compared sequence with model species R. irregularis, checked for missing genes that encode enzymes metabolic pathways related their obligate biotrophy. clarus, confirmed absence cytosolic fatty acid synthase (FAS), whereas all mitochondrial FAS components were present. A KEGG pathway map identified encoding several other two fungi, including thiamine biosynthesis conversion vitamin B6 derivatives. We also found a large proportion glucose-producing polysaccharide hydrolases, present even ectomycorrhizal appear be absent fungi. new from addition previously as missing. Missing primary imply may higher dependency on host plants than biotrophic These provide basis explore physiological characteristics auxotrophy