Transcriptome analysis and identification of genes related to terpenoid biosynthesis in Cinnamomum camphora

• DOI: 10.1186/s12864-018-4941-1
• Publication Date: 2018-07-24T05:16:05Z
• Authors: Caihui Chen; Yongjie Zheng; Yongda Zhong; Yangfang Wu; Zhiting Li; Li-An Xu; Meng Xu
• Keywords: Cinnamomum camphora; Chemotype; Borneol; Terpene; Monoterpene

Cinnamomum camphora has been cultivated as an economically important tree for its medicinal and aromatic properties. Selective breeding produced plants special uses, including spice strains with characteristic flavors aromas high-potency cultivars. The molecular biology underlying terpenoid biosynthesis is still unexplored.Gas chromatography-mass spectrometry was used to analyze the differences in contents compositions of essential oil terpenoids linalool- borneol-type chemotypes C. camphora. data revealed that oils consist primarily monoterpenes only very minor quantities sesquiterpenes diterpenes differs different camphora, higher yields (-)-borneol from than linalool-type. To study signature compounds major monoterpenes, we performed RNA sequencing profile leaf transcriptomes two A total 23.76 Gb clean generated assembled into 156,184 unigenes. length, average N50 GC content unigenes were 155,645,929 bp, 997 1430 46.5%, respectively. Among them, 76,421 annotated by publicly available databases, which 67 candidate identified be involved 2863 differentially expression between linalool-type, 1714 up-regulated 1149 down-regulated Most genes encoding proteins precursor MVA MEP pathways expressed similar levels both In addition, 10 17 DEGs significantly enriched terpene synthase activity oxidoreductase terms their directed acyclic graphs (DAG), Three monoterpene genes, TPS14-like1, TPS14-like2 TPS14-like3 compared further verified using quantitative real-time PCR.This provides a global overview gene patterns related could contribute better understanding differential accumulation chemotypes.