Regulatory motifs found in the small heat shock protein (sHSP) gene family in tomato

• DOI: 10.1186/s12864-018-5190-z
• Publication Date: 2018-12-11T04:40:24Z
• Authors: Debora Arce; Flavio Spetale; Flavia Krsticevic; Paolo Cacchiarelli; Javier De Las Rivas; Sergio Ponce; Guillermo Pratta; Elizabeth Tapia
• Keywords: Proteomics; HEAT SHOCK PROTEIN; QH426-470; Regulatory Sequences, Nucleic Acid; Gene; Applications of Calorimetry in Scientific Research; Computational biology; Solanum lycopersicum; Gene Expression Regulation, Plant; HSE; Gene Duplication; https://purl.org/becyt/ford/1.2; Protein Interaction Maps; sHSP; Promoter Regions, Genetic; Plant Proteins; 2. Zero hunger; 0303 health sciences; Regulatory motif; Biosynthesis and Engineering of Terpenoids; Heat shock protein; Life Sciences; Chemistry; Heat shock; REGULATORY MOTIF; TFBS; Physical Sciences; Molecular Chaperones in Protein Folding and Disease; Biotechnology; SHSP; Heat Shock Proteins; Tomato; 03 medical and health sciences; Biochemistry, Genetics and Molecular Biology; Genetics; Nucleotide Motifs; Physical and Theoretical Chemistry; https://purl.org/becyt/ford/1; Molecular Biology; Biology; Heat shock proteins; Research; DNA microarray; Heat-Shock Proteins, Small; FOS: Biological sciences; TOMATO; Gene expression; TP248.13-248.65; Heat-Shock Response

In living organisms, small heat shock proteins (sHSPs) are triggered in response to stress situations. This family of is large plants and, the case tomato (Solanum lycopersicum), 33 genes have been identified, most them related and ripening process. Transcriptomic proteomic studies revealed complex patterns expression for these genes. this work, we investigate coregulation by performing a computational analysis their promoter architecture find regulatory motifs known as elements (HSEs). We leverage presence sHSP members that originated from tandem duplication events analyze diversity whole family, focusing on identification HSEs.We performed search conserved genomic sequences regions sHSPs tomato, plus several other (mainly HSPs) functionally situations or ripening. Several analyses were build multiple sequence identify transcription factor binding sites (TFBS) homologous HSF1AE HSF21 Arabidopsis. also investigated interaction under two protoplast cells, both absence A2 (HsfA2). The results indicate different up-regulated depending activation repression HsfA2, key regulator HSPs. Further, protein-protein between protein (Hsp70, Hsp90 MBF1c) suggests mediating alternative through subnetwork not dependent HsfA2.Overall, study identifies (HSF1AE HSF21) associated with which considered HSEs. that, despite apparent redundancy proteins, has linked gene duplication, showed up-regulation when analyzed