RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization

0303 health sciences Transcripts degradation Gene Expression Profiling RNA Stability QH426-470 Mice, Inbred C57BL Mice 03 medical and health sciences Oogenesis Fertilization Sequence Homology, Nucleic Acid Oocyte maturation Genetics Oocytes Animals Humans Female RNA-Seq Transcriptome TP248.13-248.65 Biotechnology Research Article
DOI: 10.1186/s12864-020-06885-4 Publication Date: 2020-07-10T09:03:44Z
ABSTRACT
Abstract Background Female infertility is a worldwide concern and the etiology of has not been thoroughly demonstrated. Although mouse good model system to perform functional studies, differences between human also need be considered. The objective this study elucidate different molecular mechanisms underlying oocyte maturation fertilization mouse. Results A comparative transcriptome analysis was performed identify differentially expressed genes associated biological processes oocytes. In total, 8513 common genes, as well 15,165 6126 uniquely were detected in MII oocytes, respectively. Additionally, ratios non-homologous oocytes 37 8%, Functional categorization revealed that cAMP-mediated signaling, sister chromatid cohesin, cell recognition major enriched processes. Interestingly, we couldn’t detect any GO categories genes. Conclusions This demonstrates exhibit significant gene expression profiles during maturation, which probably deciphers differential responses fertilization. limit generalizations from maturation. Knowledge about limitations animal models crucial when exploring complex process such
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