RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization
0303 health sciences
Transcripts degradation
Gene Expression Profiling
RNA Stability
QH426-470
Mice, Inbred C57BL
Mice
03 medical and health sciences
Oogenesis
Fertilization
Sequence Homology, Nucleic Acid
Oocyte maturation
Genetics
Oocytes
Animals
Humans
Female
RNA-Seq
Transcriptome
TP248.13-248.65
Biotechnology
Research Article
DOI:
10.1186/s12864-020-06885-4
Publication Date:
2020-07-10T09:03:44Z
AUTHORS (6)
ABSTRACT
Abstract Background Female infertility is a worldwide concern and the etiology of has not been thoroughly demonstrated. Although mouse good model system to perform functional studies, differences between human also need be considered. The objective this study elucidate different molecular mechanisms underlying oocyte maturation fertilization mouse. Results A comparative transcriptome analysis was performed identify differentially expressed genes associated biological processes oocytes. In total, 8513 common genes, as well 15,165 6126 uniquely were detected in MII oocytes, respectively. Additionally, ratios non-homologous oocytes 37 8%, Functional categorization revealed that cAMP-mediated signaling, sister chromatid cohesin, cell recognition major enriched processes. Interestingly, we couldn’t detect any GO categories genes. Conclusions This demonstrates exhibit significant gene expression profiles during maturation, which probably deciphers differential responses fertilization. limit generalizations from maturation. Knowledge about limitations animal models crucial when exploring complex process such
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