DNA methylation and its effects on gene expression during primary to secondary growth in poplar stems

• DOI: 10.1186/s12864-020-06902-6
• Publication Date: 2020-07-20T12:04:22Z
• Authors: Yang Zhang; Cong Liu; He Cheng; Shuanghui Tian; Yingying Liu; Shuang Wang; Huaxin Zhang; Muhammad Saqib; Hairong Wei; Zhigang Wei
• Keywords: Bisulfite sequencing

Abstract Background As an important epigenetic mark, 5-methylcytosine (5mC) methylation is involved in many DNA-dependent biological processes and plays a role during development differentiation of multicellular organisms. However, there still lack knowledge about the dynamic aspects roles global 5mC wood formation tree trunks. In this study, we not only scrutinized single-base resolution methylomes primary stems (PS), transitional (TS), secondary (SS) Populus trichocarpa using high-throughput bisulfite sequencing technique, but also analyzed effects on expression genes formation. Results The overall average percentages CG, CHG, CHH poplar were ~ 53.6%, 37.7%, 8.5%, respectively, differences genome-wide CG/CHG/CHH contexts among PS, TS, SS statistically significant ( p < 0.05). evident 2 kb proximal promoters, gene bodies, downstream regions observed SS. Further analysis revealed perceptible correlation between levels bodies transcript failed to reveal promoter levels. We identified 653 858 DMGs 4978 4780 DEGs PS vs TS comparisons, respectively. Only 113 DEGs, 114 DEG common. Counterparts some these common other species, including Arabidopsis thaliana , are known be cell wall biosynthesis hormone signaling. This indicates that may directly modulate indirectly attune signaling genes, which turn impact Conclusions DNA marginally affects pathway or regulators formation, suggesting further studies should lean towards indirect methylation. information data provide here will instrumental for understanding species.