MicroRNA expression profile analysis in sperm reveals hsa-mir-191 as an auspicious omen of in vitro fertilization

• DOI: 10.1186/s12864-020-6570-8
• Publication Date: 2020-02-17T15:07:01Z
• Authors: Hua Xu; Xin Wang; Zhikai Wang; Jianhui Li; Zhiming Xu; Maohua Miao; Guowu Chen; Xiangdong Lei; Jun Wu; Huijuan Shi; Ke Wang; Tiancheng Zhang; Xiaoxi Sun
• Keywords: Male; 0301 basic medicine; Embryonic Development; Fertilization in Vitro; QH426-470; Mice; 03 medical and health sciences; Hsa-mir-34c; Genetics; Animals; Humans; RNA-Seq; miRNA; Gene Library; Gene Expression Profiling; Gene Expression Regulation, Developmental; High-Throughput Nucleotide Sequencing; Hsa-mir-191; Spermatozoa; MicroRNAs; ROC Curve; Embryonic development; Transcriptome; TP248.13-248.65; Biomarkers; Biotechnology; Research Article

Abstract Background MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown.

Results In this study, RNA-seq data collected from sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were analyzed for the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER). The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p < 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results.

Conclusions These findings suggest that high hsa-mir-191-5p expression in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).