Oxford Nanopore and Bionano Genomics technologies evaluation for plant structural variation detection

• DOI: 10.1186/s12864-022-08499-4
• Publication Date: 2022-04-21T18:06:02Z
• Authors: Aurélie Canaguier; Romane Guilbaud; Erwan Denis; Ghislaine Magdelenat; Caroline Belser; Benjamin Istace; Corinne Cruaud; Patrick Wincker; Marie-Christine L...; Patricia Faivre-R...; Valérie Barbe
• Keywords: Nanopore; Structural Variation; Variation (astronomy)

Structural Variations (SVs) are genomic rearrangements derived from duplication, deletion, insertion, inversion, and translocation events. In the past, SVs detection was limited to cytological approaches, then Next-Generation Sequencing (NGS) short reads partitioned assemblies. Nowadays, technologies such as DNA long read sequencing optical mapping have revolutionized understanding of in genomes, due enhancement power detection. This study aims investigate performance two techniques, 1) long-read obtained with MinION device (Oxford Nanopore Technologies) 2) Saphyr (Bionano Genomics) detect characterize genomes ecotypes Arabidopsis thaliana, Columbia-0 (Col-0) Landsberg erecta 1 (Ler-1).