Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica

• DOI: 10.1186/s12864-022-08644-z
• Publication Date: 2022-06-04T14:02:29Z
• Authors: Alison Ricafrente; Krystyna Cwiklinski; Hieu Nguyen; John P. Dalton; Nham Tran; Sheila Donnelly
• Keywords: Mammals; 0301 basic medicine; Cathepsin L3; Research; QH426-470; Fasciola hepatica; Newly excysted juvenile; microRNAs; Host-Parasite Interactions; 3. Good health; MicroRNAs; 03 medical and health sciences; Helminth; Genetics; Animals; Humans; Parasites; Transcriptome; TP248.13-248.65; Biotechnology

MiRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression in organisms ranging from viruses to mammals. There is great relevance understanding how miRNAs genes involved the growth, development, and maturation of many parasitic worms (helminths) together afflict more than 2 billion people.Here, we describe expressed by each predominant intra-mammalian development stages Fasciola hepatica, a foodborne flatworm infects wide range mammals worldwide, most importantly humans their livestock. A total 124 were profiled, 72 which had been previously reported three conserved miRNA sequences described here for first time. The remaining 49 novel which, 31 with F. gigantica 18 specific hepatica. newly excysted juveniles express 22 unique while immature liver mature bile duct 16 miRNAs. We discovered several sequence variant (IsomiRs) as well clusters exhibit strict temporal paralleling parasite development. Target analysis revealed close association between stage-specific changes transcriptome; example, identified target proteases known be essential intestinal wall penetration (cathepsin L3). Moreover, demonstrate fine-tune metabolic pathways allow parasites move an aerobic external environment anerobic host.These results provide insight into regulation helminth identifies new therapeutic limit virulence pathogenesis caused