Genome-wide analysis of the GLP gene family and overexpression of GLP1-5–1 to promote lignin accumulation during early somatic embryo development in Dimocarpus longan

• DOI: 10.1186/s12864-023-09201-y
• Publication Date: 2023-03-21T12:03:06Z
• Authors: Zhuoyun Li; Zhuoran Fu; Shuting Zhang; Xueying Zhang; Xiaodong Xue; Yukun Chen; Zihao Zhang; Zhongxiong Lai; Yuling Lin
• Keywords: 2. Zero hunger; Subcellular localization; Research; Gene Expression Profiling; Somatic embryogenesis; Hydrogen Peroxide; 15. Life on land; QH426-470; Lignin; D. longan; Sapindaceae; GLP gene family; Genetics; Expression pattern; TP248.13-248.65; Biotechnology

Longan (Dimocarpus longan Lour.) is an economically important subtropical fruit tree. Its quality and yield are affected by embryo development. As a plant seed germination marker gene, the germin-like protein (GLP) gene plays role in However, mechanism underlying of GLP somatic embryos still unclear. Therefore, we conducted genome-wide identification (DlGLP) preliminarily verified function DlGLP1-5-1. Thirty-five genes were identified as divided into 8 subfamilies. Based on transcriptome data qRT‒PCR results, DlGLP exhibited highest expression levels root, most DlGLPs was upregulated during early embryogenesis (SE) responded to high temperature stress 2,4-D treatment; eight under MeJA treatment, four them downregulated ABA treatment. Subcellular localization showed that DlGLP5-8-2 DlGLP1-5-1 located cytoplasm extracellular stroma/chloroplast, respectively. Overexpression DIGLP1-5-1 globular (GEs) promoted accumulation lignin decreased H2O2 content regulating activities ROS-related enzymes. The results provide reference for functional analysis related research improving agricultural industry through genetic engineering.