Identification of two major QTLs for pod shell thickness in peanut (Arachis hypogaea L.) using BSA-seq analysis

• DOI: 10.1186/s12864-024-10005-x
• Publication Date: 2024-01-16T10:03:04Z
• Authors: Hongfei Liu; Zheng Zheng; Ziqi Sun; Feiyan Qi; Juan Wang; Mengmeng Wang; Wenzhao Dong; Kailu Cui; Mingbo Zhao; Xiao Wang; Meng Zhang; Xiaohui Wu; Yue Wu; Dandan Luo; Bingyan Huang; Zhongxin Zhang; Gangqiang Cao; Xinyou Zhang
• Keywords: Candidate gene; Bulked segregant analysis; Arachis hypogaea

Abstract Background Pod shell thickness (PST) is an important agronomic trait of peanut because it affects the ability shells to resist pest infestations and pathogen attacks, while also influencing shelling process. However, very few studies have explored genetic basis PST. Results An F 2 segregating population derived from a cross between thick-shelled cultivar Yueyou 18 (YY18) thin-shelled Weihua 8 (WH8) was used identify quantitative loci (QTLs) for On bulked segregant analysis sequencing (BSA-seq), four QTLs were preliminarily mapped chromosomes 3, 8, 13, 18. Using genome resequencing data YY18 WH8, 22 kompetitive allele-specific PCR (KASP) markers designed genotyping population. Two major ( qPSTA08 qPSTA18 ) identified finely mapped, with detected on chromosome (0.69-Mb physical genomic region) (0.15-Mb region). Moreover, explained 31.1–32.3% 16.7–16.8% phenotypic variation, respectively. Fifteen genes in two candidate regions, including three nonsynonymous mutations exon region. molecular (Tif2_A08_31713024 Tif2_A18_7198124) that developed QTL regions effectively distinguished materials. Subsequently, validated 2:3 lines selected. Conclusions The this study may lay foundation breeding cultivars suitable mechanized shelling.