Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture

• DOI: 10.1186/s12866-014-0263-x
• Publication Date: 2014-10-22T04:53:02Z
• Authors: Flávio Krzyzanowski; Lincohn Zappelini; Solange Martone-R...; Milena Dropa; Maria Helena Matté; Flávia Nacache; Maria Tereza Pepe...
• Keywords: Sulfamethoxazole; Trimethoprim

This study aims to scrutinize Salmonella spp. and its serotypes in sewage sludge samples from wastewater treatment plants, assesses the presence of virulence genes antibiotics resistant profile. Samples (n = 54) were collected analyzed accordance with EPA Method 1682/2006. For positive serological reaction, 40 strains selected for PCR analyses detection spvC, invA sseL genes, plasmid resistance antibiotics. was detected 38.9% (<0.006473 12.19 MPN/gTS). The most prevalent serotype Infantis. All 35) presented at least one three mentioned above 40% harboured plasmids. Typhimurium isolated harbouring following genes: or sseL. Four isolates tetracycline; trimethoprim-sulfamethoxazole, isolate ciprofloxacin. Two multi antimicrobial agents. results obtained demonstrated that have been found sludge, thus it is essential set measures mitigate human health risks when intended be applied on agricultural soils.