Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads

• DOI: 10.1186/s12866-015-0615-1
• Publication Date: 2015-12-17T15:33:14Z
• Authors: Vimac Nolla-Ardèvol; Miriam Peces; Marc Strous; Halina E. Tegetmeyer
• Keywords: Biogas; Spirulina (dietary supplement); Microbial consortium

Anaerobic digestion is a biological process in which consortium of microorganisms transforms complex substrate into methane and carbon dioxide. A good understanding the interactions between populations that form this can contribute to successful anaerobic substrate. In study we combine analysis biogas production laboratory digester fed with microalgae Spirulina, protein rich substrate, metagenome responsible for digestion, obtained by high-throughput DNA sequencing. The was also compared from full scale plant cellulose material. optimal organic loading rate Spirulina determined be 4.0 g L−1 day−1 specific 350 mL −1 content 68 %. Firmicutes dominated microbial at 38 % abundance followed Bacteroidetes, Chloroflexi Thermotogae. Euryarchaeota represented 3.5 total abundance. most abundant organism (14.9 %) related Tissierella, bacterium known use proteinaceous substrates growth. Methanomicrobiales Methanosarcinales archaeal community. Compared cellulose-fed digesters, Pfam domains degradation were more frequently detected less frequent our sample. results presented suggest suitable biogas. appeared have selective impact on bacterial community performed digestion. direct influence selection methanogenic not observed.