Genome-wide transcriptome profiling of nitrogen fixation in Paenibacillus sp. WLY78

• DOI: 10.1186/s12866-016-0642-6
• Publication Date: 2016-03-01T13:42:45Z
• Authors: Hao-wen Shi; Li-ying Wang; Xin-xin Li; Xiao-meng Liu; Tian-yi Hao; Xiao-juan He; San-feng Chen
• Keywords: Paenibacillus; Transcription

Diazotrophic (nitrogen-fixing) Gram-positive and endospore-formed Paenibacillus spp. have potential uses as a bacterial fertilizer in agriculture. The transcriptional analysis of nitrogen fixation is lacking, although regulation mechanisms been well studied Gram-negative diazotrophs.Here we report global profiling sp. WLY78 cultured under N2-fixing condition (without O2 NH4(+)) non-N2-fixing (air 100 mM NH4(+)). nif (nitrogen fixation) gene operon composed 9 genes (nifBHDKENXhesAnifV) this bacterium was significantly up-regulated compared to condition, indicating that transcription strictly controlled by NH4(+) O2. qRT-PCR confirmed these were differently expressed. Non-nif specifically required fixation, such mod, feoAB cys encoding transporters Mo, Fe S atoms, coordinately transcribed with condition. transcript abundance suf specific for synthesis Fe-S cluster suggesting Sul system, which takes place nifS nifU, plays important role the nitrogenase. We discover electron might provide from protein MoFe glnR whose predicted mediate levels metabolism anaerobic respiration also analyzed.The Fur Fnr are involved GlnR This study provides valuable insights into process firmicutes.