Detection and characterization of carbapenem resistant Gram‐negative bacilli isolates recovered from hospitalized patients at Soba University Hospital, Sudan
0301 basic medicine
Sulfamethoxazole
Antibiotic resistance
Carbapenemase resistant genes
FOS: Basic medicine
Cefotaxime
Ceftazidime
Trimethoprim
Hospitals, University
Sudan
Aztreonam
Endocrinology
Antibiotics
Multidrug‐resistant
Carbapenem
Dynamics and Pathogenesis of Cholera Bacteria
Ceftriaxone
Life Sciences
Hospitalized patients
QR1-502
3. Good health
Nosocomial Pathogens: Stenotrophomonas maltophilia and Related Bacteria
Nitrofurantoin
Pseudomonas aeruginosa
Molecular Medicine
Gram negative bacteria
Research Article
Bacilli
Microbiology
beta-Lactamases
Global Challenge of Antibiotic Resistance in Bacteria
03 medical and health sciences
Bacterial Proteins
Biochemistry, Genetics and Molecular Biology
Drug Resistance, Bacterial
Gram-Negative Bacteria
Genetics
Humans
Biology
Bacteria
Amoxicillin
Clavulanic acid
Imipenem
Carbapenems
Genes, Bacterial
Antibiotic Resistance
FOS: Biological sciences
Drug resistance
Ampicillin
Gram-Negative Bacterial Infections
DOI:
10.1186/s12866-021-02133-1
Publication Date:
2021-05-04T07:03:09Z
AUTHORS (12)
ABSTRACT
Abstract
Background
Antimicrobial resistance (AMR) poses a complex threat to global health security and universal health coverage. Recently, nosocomial infections with carbapenemase-producing Gram-negative bacilli (GNB) is increasing worldwide. We report the molecular characterization and detection of genes associated with carbapenemase producing Gram negative bacteria isolated from hospitalized patients at Soba University Hospital (SUH) in Khartoum State, Sudan.
Results
Between October 2016 and February 2017, a total of 206 GNB clinical specimens were collected from hospitalized patients in SUH. Of 206 carbapenem resistance isolates, 171 (83 %) were confirmed as phenotypically resistant and 121 (58.7 %) isolates harboured one or more carbapenemase genes. New Delhi metallo-β-lactamase (NDM) types were the most predominant genes, blaNDM 107(52 %), followed by blaIMP 7 (3.4 %), blaOXA-48 5(2.4 %) and blaVIM 2 (0.9 %). Co-resistance genes with NDM producing GNB were detected in 87 (81.3 %) of all blaNDM producing isolates. NDM-1 was the most frequent subtype observed in 75 (70 %) blaNDM producing isolates. The highest percentage of resistance was recorded in ampicillin (98 %), cephalexin (93.5 %) amoxicillin clavulanic acid (90 %), cefotaxime (89.7 %), ceftriaxone (88.4 %), ceftazidime (84.2 %), sulfamethoxazole-trimethoprim (78.4 %) and nitrofurantoin (75.2 %), aztreonam (66 %) and temocillin (64 %). A close correlation between phenotypic and carbapenemase genes detection in all GNB was observed.
Conclusions
The frequency of carbapenemase producing bacilli was found to be high in SUH. NDM was found to be the most prevalent carbapenemase gene among clinical isolates. Close surveillance across all hospitals in Sudan is required. The relative distribution of carbapenemase genes among GNB in nosocomial infections in Africa needs to be defined.
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