Application of next-generation sequencing technology to study genetic diversity and identify unique SNP markers in bread wheat from Kazakhstan

• DOI: 10.1186/s12870-014-0258-7
• Publication Date: 2014-09-27T10:34:37Z
• Authors: Yuri Shavrukov; Radoslaw Suchecki; Serik Eliby; Aigul Abugalieva; Serik Kenebayev; Peter Langridge
• Keywords: Germ plasm; SNP

New SNP marker platforms offer the opportunity to investigate relationships between wheat cultivars from different regions and assess mechanism processes that have led adaptation particular production environments. Wheat breeding has a long history in Kazakhstan aim of this study was explore relationship key varieties germplasm programs for other regions. The revealed 5,898 polymorphic markers amongst ten cultivars, which 2,730 were mapped consensus genetic map. Mapped distributed almost equally across A B genomes, with 279 484 assigned each chromosome. Marker coverage approximately 10-fold lower D genome. There 863 identified as unique specific clusters these (regions containing more than three closely SNPs) showed patterns on map cultivar. Significant intra-varietal polymorphism (Tzelinnaya 3C, Kazakhstanskaya rannespelaya 15). Phylogenetic analysis based inter-varietal very old cultivar Erythrospermum 841 most genetically distinct nine Kazakhstan, falling clade together American Sonora genotypes Central South Asia. modern 19 also fell into separate clade, Thatcher. remaining eight shared single sub-clade but categorised four clusters. accumulated data polymorphisms bread may be used studying diversity wheat, potential application marker-assisted selection preparation set genotype-specific markers.