Effect of atmospheric carbon dioxide levels and nitrate fertilization on glucosinolate biosynthesis in mechanically damaged Arabidopsis plants

• DOI: 10.1186/s12870-016-0752-1
• Publication Date: 2016-03-21T20:00:57Z
• Authors: Jamuna Risal Paudel; Alexandre Amirizian; Sebastian Krosse; Jessica Giddings; Shoieb Akaram Ari...; Jianguo Xia; James B. Gloer; Nicole M. van Dam; Jacqueline C. Bede
• Keywords: 580; 0301 basic medicine; 2. Zero hunger; Nitrate fertilization; 570; Nitrates; Arabidopsis thaliana; Atmosphere; Glucosinolates; Molecular Plant Physiology; Arabidopsis; Glucosinolate; Plant Science; Carbon Dioxide; 15. Life on land; Plant Leaves; 03 medical and health sciences; Carbon dioxide; Ecogenomics; Microbiology and Immunology; 13. Climate action; Fertilizers; Research Article; Plant Diseases

Increased atmospheric carbon dioxide (CO2) levels predicted to occur before the end of the century will impact plant metabolism. In addition, nitrate availability will affect metabolism and levels of nitrogen-containing defense compounds, such as glucosinolates (GSLs). We compared Arabidopsis foliar metabolic profile in plants grown under two CO2 regimes (440 vs 880 ppm), nitrate fertilization (1 mM vs 10 mM) and in response to mechanical damage of rosette leaves.Constitutive foliar metabolites in nitrate-limited plants show distinct global patterns depending on atmospheric CO2 levels; in contrast, plants grown under higher nitrate fertilization under elevated atmospheric CO2 conditions have a unique metabolite signature. Nitrate fertilization dampens the jasmonate burst in response to wounding in plants grown at elevated CO2 levels. Leaf GSL profile mirrors the jasmonate burst; in particular, indole GSLs increase in response to damage in plants grown at ambient CO2 but only in nitrate-limited plants grown under elevated CO2 conditions.This may reflect a reduced capacity of C3 plants grown under enriched CO2 and nitrate levels to signal changes in oxidative stress and has implications for future agricultural management practices.