Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.)

• DOI: 10.1186/s12870-020-02436-x
• Publication Date: 2020-05-24T10:02:33Z
• Authors: Jianbin Shi; Ning Wang; Hong Zhou; Qinghua Xu; Gentu Yan
• Keywords: Budding

Abstract Background The axillary bud is an important index of cotton plant-type traits, and the molecular mechanism development in upland has not yet been reported. We obtained a mutant (designated mZ571) with high-budding phenotype from low-budding variety G. hirsutum Z571 (CCRI 9A02), which provided ideal materials for study complex regulatory networks development. In this study, RNA sequencing was carried out to detect gene expression levels during three stages buds (LB, low budding) mZ571 (HB, high budding). Results A total 7162 DEGs were identified groups (HB-E vs. LB-E, HB-G1 LB-G1, HB-G2 LB-G2), including 4014 downregulated 3184 upregulated DEGs. Additionally, 221 commonly all groups, accounting approximately 3.09% These identified, annotated classified. significant number related hormone metabolism, signal transduction, starch sucrose metabolism. addition, 45, 22 9 involved metabolic pathways 67, 19 transduction pathwayspathway HB-E LB-G2, respectively, suggesting that endogenous hormones are primary factors influencing growth. Hormone soluble sugar content measurements revealed exhibited higher concentrations zeatin, gibberellins stages, confirmed these metabolism-, transduction- metabolism-related genes showed interaction effects contributing divergence growth between Z571. Conclusions Our results importance sugars buds, we found plants, concentrations. Overall, present model emergence provides insights into complexity dynamic nature network