The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway

• DOI: 10.1186/s12870-020-02690-z
• Publication Date: 2020-10-16T09:03:30Z
• Authors: Heng Liu; Xiaocen Ma; Shaohua Liu; Bingyang Du; Nini Cheng; Yong Wang; Yuanhu Zhang
• Keywords: Drought Tolerance; Malondialdehyde; Osmotic shock

Drought stress is an environmental factor that limits plant growth and reproduction. Little research has been conducted to investigate the MLP gene in tobacco. Here, NtMLP423 was isolated identified, its role drought studied.Overexpression of improved tolerance tobacco, as determined by physiological analyses water loss efficiency, reactive oxygen species levels, malondialdehyde content, levels osmotic regulatory substances. Overexpression transgenic plants led greater sensitivity abscisic acid (ABA)-mediated seed germination ABA-induced stomatal closure. also regulated increasing ABA under conditions stress. Our study showed transcription level synthetic genes increased. reduced membrane damage ROS accumulation increased expression stress-related We found NtWRKY71 improve tolerance.Our results indicated NtMLP423-overexpressing tobacco via pathway.