Transcriptomic and chemical analyses to identify candidate genes involved in color variation of sainfoin flowers

• DOI: 10.1186/s12870-021-02827-8
• Publication Date: 2021-01-22T17:31:31Z
• Authors: Yu Qiao; Qiming Cheng; Yutong Zhang; Wei Yan; Fengyan Yi; Fengling Shi
• Keywords: Phenylpropanoid; KEGG; Metabolome; Flavonoid Biosynthesis; Metabolic pathway; Petal; Petunidin

Abstract Background Sainfoin ( Onobrychis viciifolia Scop) is not only a high-quality legume forage, but also nectar-producing plant. Therefore, the flower color of sainfoin an important agronomic trait, factors affecting its phenotype are still unclear. To gain insights into regulatory networks associated with metabolic pathways coloration compounds (flavonoids or anthocyanins) and identify key genes, we conducted comprehensive analysis phenotype, metabolome transcriptome WF AF sainfoin. Results Delphinidin, petunidin malvidin derivatives were main anthocyanin in These substances detected The transcriptomes at S1 S3 stages obtained using Illumina HiSeq4000 platform. Overall, 10,166 (4273 upregulated 5893 downregulated) 15,334 (8174 7160 DEGs identified flowers stages, respectively (WF-VS-AF). KEGG pathway annotations showed that 6396 unigenes annotated to 120 contained 866 131 included 1546 stage. Nine belonging “flavonoid biosynthesis”and “phenylpropanoid biosynthesis” involved formation verified by RT-qPCR analyses. Among these DEGs, 4CL3 , FLS ANS CHS DFR CHI2 exhibited downregulated expression, F3H expression compared AF, resulting decrease synthesis Conclusions This study first use technology mechanism white Our data will be great enrichment genetic information for In addition, presented herein provide valuable molecular breeding insight future polymorphisms