Characterization of non-specific lipid transfer protein (nsLtp) gene families in the Brassica napus pangenome reveals abundance variation

• DOI: 10.1186/s12870-021-03408-5
• Publication Date: 2022-01-07T06:07:59Z
• Authors: Yu Liang; Yang Huang; Kang Chen; Xiangdong Kong; Maoteng Li
• Keywords: Biotic stress

Brassica napus is an important agricultural species, improving stress resistance was one of the main breeding goals at present. Non-specific lipid transfer proteins (nsLTPs) are small, basic which involved in some biotic or abiotic responses. B. susceptible to a variety fungal diseases, so identify BnLTPs and their expression disease responses very important. The common reference genome does not contain all genes because gene presence/absence variations between individuals. Therefore, it necessary search for candidate BnLTP pangenome.In present study, were identified throughout pangenome, different presented among varieties. Totally, 246 could be divided into five types (1, 2, C, D, G). classification, phylogenetic reconstruction, chromosome distribution, functional annotation, analyzed. We also potential cis-elements that respond stresses 2 kb upstream regions genes. RNA sequencing analysis showed response Sclerotinia sclerotiorum infection. 32 linked blackleg quantitative trait locus (QTL).The identification LTP pangenome help elucidate function family members provide new information future molecular napus.