Comparative transcriptomic analyses of citrus cold-resistant vs. sensitive rootstocks might suggest a relevant role of ABA signaling in triggering cold scion adaption

• DOI: 10.1186/s12870-022-03578-w
• Publication Date: 2022-04-22T01:02:58Z
• Authors: Amparo Primo-Capella; María Ángeles For...; Mary-Rus Martínez...; Javier Terol
• Keywords: Citrus × sinensis; Cold stress

The citrus genus comprises a number of sensitive tropical and subtropical species to cold stress, which limits global distribution certain latitudes causes major economic loss. We used RNA-Seq technology analyze changes in the transcriptome Valencia delta seedless orange response long-term stress grafted on two frequently rootstocks: Carrizo citrange (CAR), considered one most cold-tolerant accessions; C. macrophylla (MAC), very one. Our objectives were identify genetic mechanism that produce tolerant or phenotypes citrus, as well gain insights rootstock-scion interactions induce tolerance sensitivity scion.Plants kept at 1 ºC for 30 days. Samples taken 0, 15 metabolomic analysis showed significant increase concentration free sugars proline, was higher CAR plants. Hormone quantification roots substantially increased ABA during exposure roots, not observed MAC. Different approaches followed gene expression. During treatment, 0-15-day comparison yielded DEGs. functional characterization DEGs enrichment GO terms KEGG pathways related abiotic responses previously described plant adaption. revealed several key genes promoting adaption up-regulated plants, those repressing it had expression levels MAC samples.The transcriptomic study herein performed indicates mechanisms activated plants shortly after remain active long term. Both hormone differential suggest signaling might play relevant role hardiness sensitiveness sweet onto Macrophylla rootstocks, respectively. work provides new into by rootstocks modulate resistance production variety them.