Suppression of microRNA168 enhances salt tolerance in rice (Oryza sativa L.)
0301 basic medicine
2. Zero hunger
miR168
Research
Salt stress
Botany
Oryza
Salt Tolerance
15. Life on land
Salt Stress
MicroRNAs
03 medical and health sciences
Seedlings
QK1-989
Rice
STTM
Gene regulation cascades
DOI:
10.1186/s12870-022-03959-1
Publication Date:
2022-12-03T02:02:23Z
AUTHORS (14)
ABSTRACT
Abstract
Background
Rice is a salt-sensitive crop. Complex gene regulatory cascades are likely involved in salinity stress in rice roots. microRNA168 (miR168) is a conserved miRNA among different plant species. It in-directly regulates the expression of all miRNAs by targeting gene ARGONAUTE1(AGO1). Short Tandem Target Mimic (STTM) technology is an ideal approach to study miRNA functions by in-activating mature miRNA in plants.
Results
In this study, rice miR168 was inactivated by STTM. The T3 generation seedlings of STTM168 exhibited significantly enhanced salt resistance. Direct target genes of rice miR168 were obtained by in silico prediction and further confirmed by degradome-sequencing. PINHEAD (OsAGO1), which was previously suggested to be a plant abiotic stress response regulator. RNA-Seq was performed in root samples of 150mM salt-treated STTM168 and control seedlings. Among these screened 481 differentially expressed genes within STTM168 and the control, 44 abiotic stress response related genes showed significant difference, including four known salt-responsive genes.
Conclusion
Based on sequencing and qRT-PCR, a “miR168-AGO1-downstream” gene regulation model was proposed to be responsible for rice salt stress response. The present study proved miR168-AGO1 cascade to play important role in rice salinity stress responding, as well as to be applied in agronomic improvement in further.
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