Transcriptomics and metabolomics reveal the underlying mechanism of drought treatment on anthocyanin accumulation in postharvest blood orange fruit

• DOI: 10.1186/s12870-024-04868-1
• Publication Date: 2024-03-02T03:01:36Z
• Authors: Hongbin Liu; Yan Jin; Le Huang; Chouyu Miao; Jiayi Tang; Huimin Zhang; Haojie Yin; Xiaopeng Lu; Na Li; Suming Dai; Alessandra Gentile; Ling Zhang; Ling Sheng
• Keywords: Metabolome

Abstract Background Anthocyanins are the most important compounds for nutritional quality and economic values of blood orange. However, there few reports on pre-harvest treatment accelerating accumulation anthocyanins in postharvest orange fruit. Here, we performed a comparative transcriptome metabolomics analysis to elucidate underlying mechanism involved seasonal drought (SD) during fruit expansion stage anthocyanin ‘Tarocco’ Results Our results showed that SD slowed down enlargement increased sugar development maturation period. Obviously, under treatment, storage was significantly accelerated markedly higher than CK. Meanwhile, total flavonoids phenols content antioxidant activity fruits were also sensibly storage. Based metabolome analysis, found substrates required biosynthesis, such as amino acids their derivatives, phenolic acids, had accumulated treated mature compared with Furthermore, according data weighted gene coexpression correlation network (WGCNA) phenylalanine ammonia-lyase ( PAL3 ) considered key structural gene. The qRT-PCR verified highly expressed stored fruits, positively correlated content. Moreover, other genes biosynthesis pathway upregulated evidenced by analysis. Conclusions findings suggest promotes necessary ripening process, activates expression This is especially true , which co-contributed rapid anthocyanin. present study provides theoretical basis control water-saving utilization