Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/β-catenin signaling pathway

• DOI: 10.1186/s12872-020-01400-9
• Publication Date: 2020-03-05T17:02:42Z
• Authors: Wei Liu; Xiujuan Chen; Min Wu; Lin Li; Jiani Liu; Jing Shi; Tian Hong
• Keywords: Foam cell; CD36; THP1 cell line; Scavenger Receptor

Abstract Background Atherosclerosis (AS) is the basis of cardiovascular diseases, characterized by chronic inflammatory and lipid metabolism disorders. Although anti-inflammatory effect Klotho in AS has been clearly shown, its lipid-lowering unclear. In this study, we examined effects recombinant (Re-KL) protein on accumulation foam cells. Methods THP-1 cells were exposed to 100 nM phorbol myristate acetate for 24 h then oxidized low-density lipoprotein (ox-LDL; 80 mg/mL) induce cell formation. Subsequently, incubated with Re-KL and/or DKK1, an inhibitor Wnt/β-catenin pathway. Results Oil red O staining cholesterol intake assay revealed that model was constructed successfully. Pre-treatment decreased total level, up-regulated expression ATP binding cassette transporter A1 (ABCA1) G1 (ABCG1), down-regulated acyl coenzyme a-cholesterol acyltransferase 1 (ACAT1) members scavenger family (SR-A1 CD36). addition, pathway-related proteins significantly stimulus Re-KL. Interestingly, similar DKK1 Conclusions The Re-KL-induced up-regulation reverse transport capacity promotes efflux reduces suppressing pathway