Elucidating the role of circNFIB in myocardial fibrosis alleviation by endogenous sulfur dioxide

• DOI: 10.1186/s12872-022-02909-x
• Publication Date: 2022-11-20T22:02:28Z
• Authors: Jia Liu; Ranran Zhang; Dahai Wang; Yi Lin; Cui Bai; Nana Nie; Shan Gao; Qiuye Zhang; Hong Chang; Chongmin Ren
• Keywords: Angiology

Abstract Background To investigate the role of circNFIB in alleviation myocardial fibrosis by endogenous sulfur dioxide (SO 2 ). Methods We stimulated cultured neonatal rat cardiac fibroblasts with transforming growth factor-β1 (TGF-β1) and developed an vitro model. Lentivirus vectors containing aspartate aminotransferase 1 (AAT1) cDNA were used to overexpress AAT1, siRNA was silence circNFIB. The SO , collagen, circNFIB, Wnt/β-catenin, p38 MAPK pathways examined each group. Results In TGF-β1-induced model, /AAT1 expression significantly decreased, collagen levels cell supernatant type I III expression, as well α-SMA all increased. TGF-β1 also reduced expression. AAT1 overexpression while increasing Endogenous alleviated after blocked. discovered that plays important inhibiting Wnt/β-catenin pathways. Conclusion promotes which inhibits signaling pathways, consequently alleviating fibrosis.