Co-infection with dual Streptococcus pneumoniae serotypes as a cause of pediatric bacterial meningitis in Iran: a multi-center cross-sectional study

• DOI: 10.1186/s12879-022-07606-w
• Publication Date: 2022-07-18T07:02:44Z
• Authors: Sedigheh Rafiei T...; Ahmadreza Shamshiri; Leila Azimi; Ali Nazari-Alam; Abdollah Karimi; Seyed Alireza Mir...; Marjan Tariverdi
• Keywords: Medical microbiology

Abstract Background Meningitis is considered a life-threatening infection with high mortality all over the world. Hemophilus influenzae ( H. ) and Streptococcus pneumoniae S. are regarded as two most common infectious agents causing bacterial meningitis. This study aimed to identify serotypes in blood cerebrospinal fluid (CSF) of pediatric patients meningitis, using polymerase chain reaction (PCR). Methods multi-center cross-sectional included 284 children suspected meningitis referred 4 target hospitals. Overall, 412 samples (128 CSF samples) were obtained from November 14, 2016 15, 2017. The extracted DNA was examined multiplex real time PCR screen for . serotyping also done by PCR. Results Out specimens, 22 positive ply Of 20 meeting Quality Control (QC) standards serotyping, 7 (35%), 6 (30%), 2 (10%), 1 (5%), (5%) 3, 11A, 6A, 7C, 23F, 23B, 19A, 19F 5, respectively. nine serotypes, whom 3 11A Tehran province. note, one these showed new co-infection 7C 14. Also, (30%) detected bexA primer. None or Conclusion Co-infection can occur it might be missed if not evaluated specimens.