Evaluation of the multiplex PCR combined with capillary electrophoresis technique for detecting pathogenic bacteria and antibiotic resistance genes in bone infections

• DOI: 10.1186/s12879-025-10847-0
• Publication Date: 2025-04-01T19:14:27Z
• Authors: Junye Liu; Yan Yu; Wei Feng; Zhihao He; Mengfei Wang; Weikun Hou; Yannan Zhao; Yi Liu; Yuzhu Yan; Heping Zhao
• Keywords: Medical microbiology; Parasitology; Multiplex; Pathogenic bacteria

Orthopedic wound infection is a difficult problem in the clinic. Accurate and rapid microbiological test results are essential for case management, antibiotic therapy, control. We retrospectively evaluated 1285 specimens (puncture fluid, catheter, secretions, joint lavage extraction blood culture, drainage cerebrospinal bone, prosthesis tissue, etc.) from 739 patients who received orthopedic diagnosis treatment, using routine conventional method (RCM)s as reference to evaluate performance of multiplex PCR combined with capillary electrophoresis (mPCR-CE) detecting pathogens resistance genes associated bone infection. Among samples analyzed, 1045 were consistent RCM, an agreement rate 81.32%. 155 inconsistent results, 13 (1.01%) mPCR-CE negative but RCM positive, 142 (11.05%) was positive negative. Compared demonstrated percentage values 65.37% 98.35%, respectively. Moreover, detection multidrug-resistant bacteria by generally better than that method. The methicillin-resistant Staphylococcus aureus (MRSA) relatively high. traditional drug-sensitive culture more inclined detect extended-spectrum β-lactamases (ESBLs). has obvious advantages terms timeliness. This study revealed new effective diagnostic can significantly reduce identification time bacterial drug resistance, potential improve management infections.