In vitro elution characteristics of gentamicin-impregnated Polymethylmethacrylate: premixed with a second powder vs. liquid Lyophilization

• DOI: 10.1186/s12891-020-03923-w
• Publication Date: 2021-01-04T16:06:37Z
• Authors: Wongthawat Liawru...; Suwipa Ungphaiboon; Arnurai Jitsurong; Natnicha Ingviya; Boonsin Tangtraku...; Varah Yuenyongviwat
• Keywords: Bone cement

Abstract Background Antibiotic-loaded bone cement, or antibiotic-impregnated polymethylmethacrylate (PMMA), were developed to prevent and treat joint infections. Gentamicin is an antibiotic that commonly used in combination with PMMA; however, gentamicin powder hard obtain many countries. This study aimed evaluate the elution characteristics of gentamicin-impregnated PMMA made lyophilized liquid gentamicin, compared which from commercial powder. Methods The experimental sample was divided into 2 groups: power group (PG-PMMA) (LG-PMMA). Ten cement spacers prepared each group. These produced by mixing powder, a polymer before adding monomer (2 g 40 PMMA). volume surface area 50 cm 3 110 , respectively. Each spacer immersed phosphate-buffered saline, changed daily under sterile conditions. solutions collected measure level using enzyme multiplied immunoassay technique (EMIT), at days 1, 2, 3, 4, 5, 6, 7, 14, 21, 28, 35 42. Results collections both groups had high concentrations on day 1 (113.63 ± 23.42 mg/dL LG-PMMA 61.7 ±8.37 PG-PMMA), but experienced continuous decrease over time. could release for up 6 weeks (3.28 1.17 1.21 0.28 PG-PMMA). However, there significantly higher levels PG-PMMA all time points ( P < 0.05). Conclusion Gentamicin-impregnated approximately two times rate preliminary vitro studies, as premixed