Identification of key genes and long non‑coding RNA expression profiles in osteoporosis with rheumatoid arthritis based on bioinformatics analysis

• DOI: 10.1186/s12891-024-07738-x
• Publication Date: 2024-08-08T17:02:21Z
• Authors: Jin-yu An; Xing-na Ma; Hui-long Wen; Hui-dong Hu
• Keywords: Pathogenesis; Identification

Although rheumatoid arthritis (RA) is a chronic systemic tissue disease often accompanied by osteoporosis (OP), the molecular mechanisms underlying this association remain unclear. This study aimed to elucidate pathogenesis of RA and OP identifying differentially expressed mRNAs (DEmRNAs) long non-coding RNAs (lncRNAs) using bioinformatics approach. Expression profiles individuals diagnosed with were retrieved from Gene Omnibus database. Differential expression analysis was conducted. ontology (GO) Kyoto Encyclopedia Genes Genomes pathway (KEGG) enrichment analyses performed gain insights into functional categories molecular/biochemical pathways associated DEmRNAs. We identified intersection common DEmRNAs lncRNAs constructed protein-protein interaction (PPI) network. Correlation between facilitated construction coding-non-coding Lastly, serum peripheral blood mononuclear cells (PBMCs) patients OP, as well healthy controls, obtained for TRAP staining qRT-PCR validate findings online dataset assessments. A total 28 2 DElncRNAs in both OP. Chromosomal distribution consensus revealed that chromosome 1 had highest number differential genes. GO KEGG indicated these primarily " platelets (PLTs) degranulation", "platelet alpha granules", activation", "tight junctions" "leukocyte transendothelial migration", many genes functionally related PLTs. In PPI network, MT-ATP6 PTGS1 emerged potential hub genes, originating mitochondrial DNA. Co-expression two key lncRNA-mRNA pairs: RP11 − 815J21.2 MT ATP6 PTGS1. Experimental validation confirmed significant RP11-815J21.2, controls + groups. Notably, knockdown RP11-815J21.2 attenuated TNF IL-6-induced osteoclastogenesis. successfully shared dysregulated therapeutic targets highlighting their similarities. These provide new suggest avenues further research targeted therapies.