Sonication versus the conventional method for evaluation of the dental microbiome: a prospective pilot study

• DOI: 10.1186/s12903-022-02374-0
• Publication Date: 2022-08-12T16:02:54Z
• Authors: Oliver Wagendorf; Peter Menzel; Rolf Schwarzer; Norbert Neckel; Saskia Preissner; Max Heiland; Susanne Nahles
• Keywords: Sonication

Abstract Objectives To investigate sonication as a new tool in microbiological probing of dental infections. Methods Comparison standard method: intraoperative swab, with sonication, and vortex the removed tooth, was performed on 20 carious destructed teeth. Illumina high throughput sequencing 16S-rRNA-gene used for assessing microbial composition. Antibiotic susceptibility has been assigned based known resistances each detected species. Probing procedures were compared using Bland–Altmann-Test, antibiotic Friedmann-Test alpha-adjusted post-hoc-analysis. Results In total, 60 samples analysed: swabs, fluids, fluids. Sonication fluid yielded highest number bacterial reads all three procedures. Comparing operational taxonomic units (OTUs) identified bacteria, significantly more OTUs found samples. Phylum order abundances varied between Significantly Actinomycetales have to swab The resistance rates bacteria (1.79–31.23%) showed no differences tested lowest amoxicillin + clavulanate (3.95%) levofloxacin (3.40%), (30.21%) clindamycin (21.88%). Conclusions By extracted teeth, it is possible get comprehensive image residing flora procedure. If not available, vortexing potential alternative. immunocompromised patients, especially when actinomycosis suspected, should be considered detailed evaluation disease-causing microbiome. Due resistance, targeted therapy favourable. Levofloxacin first-line alternative patients an allergy penicillin.