RETRACTED ARTICLE: Xia-yu-xue decoction (XYXD) reduces carbon tetrachloride (CCl4)-induced liver fibrosis through inhibition hepatic stellate cell activation by targeting NF-κB and TGF-β1 signaling pathways

• DOI: 10.1186/s12906-015-0733-1
• Publication Date: 2015-06-29T10:18:30Z
• Authors: Cheng Liu; Xia Yuan; Le Tao; Zhuoan Cheng; Xiuqin Dai; Xia Sheng; Dongying Xue
• Keywords: Hepatic stellate cell; CCL4; Decoction; Hepatic fibrosis

Hepatic stellate cell (HSC) activation is activated mainly by endotoxin and transforming growth factor (TGF-β1) in chronic liver injury, consequently, can be important therapeutic targets. Xia-yu-xue decoction (XYXD), a classical recipe used China to treat fibrosis, has been revealed inhibit hepatic fibrosis animal models, the mechanism of action XYXD remains elusive. In present study, we evaluated whether reduced pro-fibrogenic pathways induced lipopolysaccharide (LPS) TGF-β1 HSCs.The vivo effect on progression was assessed mice model carbon tetrachloride (CCl4), The vitro GFP-Col-HSC cells using LPS stimulation.XYXD treatment CCl4-induced decreased hydroxyproline (Hyp) content, mRNA levels smooth muscle actin (α-SMA) Col 1(α1) fibrotic liver. suppressed nuclear factor-κB (NF-κB) NF-κB-luciferase reporter. expression NF-κB target genes, chemokine (C-C motif) ligand 2 (CCL2) (C-X-C (CXCL2) after treatment. targets Col1(α1) tissue inhibitor metalloproteinases (TIMP1) treatment.XYXD attenuates inhibiting HSC via inhibition signaling pathway, thereby blocking synthesis Col1 (α1) TIMP-1. These findings from study suggest that may for which are thought take part.