Anti-lipoapoptotic effects of Alisma orientalis extract on non-esterified fatty acid-induced HepG2 cells

• DOI: 10.1186/s12906-016-1181-2
• Publication Date: 2016-07-25T09:43:11Z
• Authors: Hyeon-Soo Jeong; Young-Hwan Cho; Kang-Hoon Kim; Yumi Kim; Ki-Suk Kim; Yun-Cheol Na; Jiyoung Park; In-Seung Lee; Jang-Hoon Lee; Hyeung-Jin Jang
• Keywords: Steatosis; Lipogenesis; Oil Red O

Liver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line commonly used model. The cells were cultured NEFAs mixture (oleic and palmitic acids, 2:1 ratio) 24 h to induce hepatic steatosis. Then different doses extract (AOE) treated h. Incubated further experiments. AOE showed inhibitory effects on Oil Red O staining Nile red tests no cytotoxicity at concentration 300 μg/mL. Fatty acid synthase (FASN) acetyl-CoA carboxylase 1 (ACC1) mRNA protein expression level down-regulated after treatment. Bcl-2 associated X (Bax) c-Jun N-terminal kinase (JNK) decreased well p-JNK (activated form JNK), Bax, cleaved caspase-9, caspase-3 level. Anti-apopototic B-cell lymphoma 2 (Bcl-2) increased In addition, inflammatory including p-p65, p65, COX-2 iNOS inhibited results suggest that has anti-steatosis involve lipogenesis, anti-lipoapoptosis, anti-inflammation NEFA-induced NAFLD pathological