Antituberculosis activity, phytochemical identification of Costus speciosus (J. Koenig) Sm., Cymbopogon citratus (DC. Ex Nees) Stapf., and Tabernaemontana coronaria (L.) Willd. and their effects on the growth kinetics and cellular integrity of Mycobacterium tuberculosis H37Rv

• DOI: 10.1186/s12906-017-2077-5
• Publication Date: 2018-01-08T13:02:50Z
• Authors: Suriyati Mohamad; Nur Najihah Ismail; Thaigarajan Parum...; Pazilah Ibrahim; Hasnah Osman; Habibah A. Wahab
• Keywords: Phytochemical; Cymbopogon citratus; Minimum bactericidal concentration

Costus speciosus, Cymbopogon citratus, and Tabernaemontana coronaria are herbal plants traditionally used as remedies for symptoms of tuberculosis (TB) including cough. The aims the present study were to evaluate in vitro anti-TB activity different solvent partitions these plants, identify phytochemical compounds, assess effects most active on growth kinetics cellular integrity tubercle organism.The plant materials was determined against M. H37Rv using a tetrazolium colorimetric microdilution assay. compounds partition each identified gas chromatography-mass spectrometry (GC-MS) analysis. mycobacteria evaluated over 7-day treatment period batch culture system. Their mycobacterial observed under scanning electron microscope (SEM).The respective n-hexane C. T. exhibited highest with minimum inhibitory concentrations (MICs) 100-200 μg/mL bactericidal concentration (MBC) 200 μg/mL. GC-MS analysis revealed that majority belonged lipophilic fatty acid groups. speciosus high cidal relation time, killing more than 99% cell population. SEM observations showed caused multiple structural changes indicating massive damages.The promising H37Rv. supported by their destructive structure.