The antioxidant and neurochemical activity of Apium graveolens L. and its ameliorative effect on MPTP-induced Parkinson-like symptoms in mice

• DOI: 10.1186/s12906-018-2166-0
• Publication Date: 2018-04-04T03:24:10Z
• Authors: Pennapa Chonpatho...; Phetcharat Boonru...; Wanida Sukketsiri; Pilaiwanwadee Hut...; Morakot Sroyraya
• Keywords: MPTP; Apium graveolens; Monoamine oxidase B

Apium graveolens L. is a traditional Chinese medicine prescribed as treatment for hypertension, gout, and diabetes. This study aimed to determine the neuroprotective effects of A. extract against Parkinson's disease (PD) model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6 mice. Male mice treated with MPTP were orally dosed daily 21 days. Behavioral tests, including rotarod apparatus, narrow beam test, drag grid walk swimming resting tremor evaluation, performed. Thereafter, sacrificed, monoamine oxidase A B activity, lipid peroxidation superoxide anion levels measured. Immunohistochemical staining tyrosine hydroxylase was performed identify dopaminergic neurons. We found that at dose 375 mg/kg demonstrated highest effect led significant improvements behavioral performance, oxidative stress parameters, activity compared untreated group (p < 0.05). Moreover, increased number neurons immunopositive expression alone or positive control drug speculated ameliorated performance mediating neuroprotection MPTP-induced PD via antioxidant effects, related neurotransmitter pathways an increase