Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

• DOI: 10.1186/s12906-018-2246-1
• Publication Date: 2018-06-15T01:49:54Z
• Authors: Parvaneh Mehrbod; Muna Ali Abdalla; Fatemeh Fotouhi; Masoumeh Heidarzadeh; Abimbola O. Aro; Jacobus N. Eloff; Lyndy J. McGaw; Folorunso O. Fasina
• Keywords: 0301 basic medicine; SDG-03: Good health and well-being; Rapanea melanophloeos (RM); DNA Copy Number Variations; Antiviral Agents; Madin Darby Canine Kidney Cells; Viral Matrix Proteins; Other systems of medicine; 03 medical and health sciences; Dogs; Animals; Immunologic Factors; Glycosides; Cytokine; Rapanea melanophloeos; Primulaceae; Plant Extracts; Viral Core Proteins; RNA-Binding Proteins; Quercetin-3-O-α-L-rhamnopyranoside; Immunomodulatory properties; Nucleocapsid Proteins; Influenza a virus; 3. Good health; Influenza A virus; Cytokines; Quercetin; Glycoside flavone; Veterinary science articles SDG-03; RZ201-999; Research Article

Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases this disease widely recognized. Drug resistance and side effects chemical treatments have been observed, resulting increased interest alternative use herbal medications for prophylaxis against infection. South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez the family Myrsinaceae was selected owing to its traditional treatment several diseases such as respiratory ailments also previous preliminary studies anti-influenza activity methanolic extract. aim study investigate immunomodulatory properties glycoside flavone isolated from RM virus. non-cytotoxic concentration quercetin-3-O-α-L-rhamnopyranoside (Q3R) determined by MTT assay tested virus (IAV) simultaneous, pre-penetration post-penetration combination over 1 h incubation on MDCK cells. titer viral load targeting NP M2 genes were using HA qPCR, respectively. TNF-α IL-27 pro- anti-inflammatory cytokines measured at RNA protein levels qPCR ELISA, Quercetin-3-O-α-L-rhamnopyranoside 150 μg/ml decreased 6 logs (p < 0.01) simultaneous procedure. copy numbers target genes, calculated based Ct values standard formula, significantly 0.01). expression considerably affected compound treatment. This first report Further research will focus detecting specific mechanism virus-host interactions.