Fractions and isolated compounds from Oxyanthus speciosus subsp. stenocarpus (Rubiaceae) have promising antimycobacterial and intracellular activity

• DOI: 10.1186/s12906-019-2520-x
• Publication Date: 2019-05-22T15:06:37Z
• Authors: Abimbola O. Aro; Jean P. Dzoyem; Maurice D. Awouafack; Mamoalosi A. Selepe; Jacobus N. Eloff; Lyndy J. McGaw
• Keywords: Antimycobacterial

Tuberculosis is a deadly disease caused by Mycobacterium species. The use of medicinal plants an ancient global practice for the treatment and prevention diverse ailments including tuberculosis. aim this study was to isolate characterize antimycobacterial compounds bioassay-guided fractionation acetone leaf extract Oxyanthus speciosus. A two-fold serial microdilution method used determine minimum inhibitory concentration (MIC) against mycobacteria. Cytotoxicity nitric oxide activity isolated determined evaluate in vitro safety potential anti-inflammatory activity. Intracellular efficacy crude Mycobacterium-infected macrophages also determined. Two were identified as lutein (1) rotundic acid (2). These had good four mycobacteria tested with MIC values ranging from 0.013 0.1 mg/mL. Rotundic some cytotoxicity C3A human liver cells. Lutein not cytotoxic at highest (200 μg/mL) inhibited production RAW 264.7 94% 25 μg/mL. (120 O. speciosus intracellular activity, reducing colony forming units more than 90%, displaying bactericidal dose time-dependent manner. This provides proof presence synergism between different extracts fractions. It first report promising both intracellularly macrophage model suggests its development anti- tuberculosis (TB) herbal medicine.