Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans

• DOI: 10.1186/s12906-019-2626-1
• Publication Date: 2019-08-13T12:03:08Z
• Authors: Palika Wetchakul; Jo Aan Goon; Ademola Ezekiel A...; Opeyemi Joshua Ol...; Sutticha Ruangchuay; Patcharawalai Jai...; Acharaporn Issuriya; Nongluk Kunworarath; Surasak Limsuwan; Sasitorn Chusri
• Keywords: Herbal tonic; Aging; Longevity; Phyllanthus emblica; Gamma-Aminobutyric Acid Metabolism in Plants; Plant Science; Biochemistry; Gene; Antioxidants; Agricultural and Biological Sciences; Other systems of medicine; 03 medical and health sciences; Glucosides; Phenols; Biochemistry, Genetics and Molecular Biology; Health Sciences; Animals; Molecular Mechanisms of Aging and Longevity; Benzopyrans; Caenorhabditis elegans; Biology; Medicine, East Asian Traditional; Pharmacology; 0303 health sciences; Plant Extracts; Herbal Medicine for Neurological Disorders; Life Sciences; Antioxidant Activity; Traditional medicine; Thailand; 6. Clean water; Polyherbal formula; Oxidative Stress; Complementary and alternative medicine; Tonifying agents; Terminalia; Medicine; Antioxidant; RZ201-999; Research Article

The imbalance between the generation of free radicals and natural cellular antioxidant defenses, known as oxidative stress, can cause oxidation biomolecules further contribute to aging-associated diseases. purpose this study was evaluate capacities Thai traditional tonifying preparation, Jatu-Phala-Tiga (JPT) its herbal ingredients consisting Phyllanthus emblica, Terminalia arjuna, chebula, bellirica assess effect on longevity.Antioxidant activities various extracts obtained from JPT components were carried out using well-established methods including metal chelating, radical scavenging, ferric reducing power assays. Qualitative analysis chemical composition water extract done by high-performance liquid chromatography tandem with electrospray ionisation mass spectrometry. lifespan Caenorhabditis elegans additionally described.Among extracts, exerted remarkable compared other solvents individual constituting plant extract. found possess highest chelating activity, an IC50 value 1.75 ± 0.05 mg/mL. Moreover, it exhibited scavenging towards DPPH, ABTS, superoxide anion radicals, values 0.31 0.02, 0.308 0.004, 0.055 0.002 mg/mL, respectively. ORAC FRAP 40.338 2.273 μM Trolox/μg 23.07 1.84 mM FeSO4/mg sample, Several well-known antioxidant-related compounds amaronols, quinic acid, gallic fertaric kurigalin, amlaic isoterchebin, chebulagic ginkgolide C, chebulinic ellagic rutin in Treatment at 1 5 mg/mL increased C. under normal growth condition (7.26 0.65 vs. 10.4 0± 0.75 (p < 0.01) 10.00 0.73 days, respectively).The results indicated that strong activities, particular polyherbal tonic. These findings rationalize investigation infusion a promising agent for anti-aging stress prevention.