Taohuajing reduces oxidative stress and inflammation in diabetic cardiomyopathy through the sirtuin 1/nucleotide-binding oligomerization domain-like receptor protein 3 pathway

• DOI: 10.1186/s12906-021-03218-0
• Publication Date: 2021-02-26T13:03:39Z
• Authors: Rui Yao; Yu Cao; Changming Wang; Lu Xu; Xuan Zhang; Yuqing Deng; Feng Li; Siwang Wang
• Keywords: Diabetic Cardiomyopathy; Malondialdehyde

Abstract Background Oxidative stress and inflammation promote the development of diabetic cardiomyopathy (DCM). Therefore, inhibiting these processes may show beneficial effects in treatment patients with DCM. Taohuajing (THJ) is prepared using Persicae semen (Taoren), Polygonatum sibiricum (Huangjing), Carthami flos (Honghua) have applications However, protective THJ not been thoroughly assessed. Accordingly, this study, we aimed to investigate a model DCM further clarify potential mechanisms. Methods A type 2 diabetes mellitus was generated male C57BL/6 mice. Echocardiography histopathology were used evaluate cardiac function. The expression levels cytokines measured enzyme-linked immunosorbent assays. Western blotting small interfering RNA targets THJ. Results Compared control group, mice showed dysfunction, metabolic disorder, fibrosis, disorganized ultrastructure, significantly inhibited changes significantly. also production reactive oxygen species (ROS) malondialdehyde (MDA), induced glutathione peroxidase (GSH-Px) superoxide dismutase (SOD), decreased pro-inflammatory cytokines, suppressed activation nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome. These abolished by sirtinol, an inhibitor sirtuin1 (SIRT1). Conclusions Overall, protected heart from hyperglycemia-induced oxidative via mechanism involving SIRT1-mediated antioxidant proteins suppression NLRP3