Myrtenal and β-caryophyllene oxide screened from Liquidambaris Fructus suppress NLRP3 inflammasome components in rheumatoid arthritis

• DOI: 10.1186/s12906-021-03410-2
• Publication Date: 2021-09-28T14:02:50Z
• Authors: Wen-xuan Li; Ping Qian; Yi-tong Guo; Li Gu; Jessore Jurat; Yang Bai; Dong-fang Zhang
• Keywords: β-Caryophyllene oxide; Male; Polycyclic Sesquiterpenes; 0301 basic medicine; Inflammasomes; Research; Rats, Inbred WF; Network Pharmacology; NLRP3 inflammasome; Rats; 3. Good health; Arthritis, Rheumatoid; Molecular Docking Simulation; Other systems of medicine; 03 medical and health sciences; Myrtenal; NLR Family, Pyrin Domain-Containing 3 Protein; Animals; Rheumatoid arthritis; Liquidambaris Fructus; RZ201-999; Bicyclic Monoterpenes

Abstract Background Liquidambaris Fructus (LF) is the infructescence of Liquidambar formosana . In Traditional Chinese Medicine, LF has been used to treat joint pain, a common symptom arthritis and rheumatism; however, lack pharmacological evidence limited its applications in modern clinics. Therefore, this study aims explore protective effect on rheumatoid (RA) identify active ingredients. Methods Rats with adjuvant-induced (AIA) were divided into 4 groups administered petroleum ether extract (PEL), ethyl acetate (EEL), water (WEL), or piroxicam (PIR) respectively for 3 weeks. Two additional as normal control (NC) model (MC) distilled placebo. The clinical scores arthritis, bone surface, synovial inflammation cartilage erosion evaluate therapeutic efficacy each treatment. serum IL-1β TNF-α level expression NLRP3, caspase-1 p20 tissue AIA rats evaluated by ELISA Western blot. ingredients investigated using network pharmacology molecular docking methods, their inhibition NLRP3 inflammasome activation was verified human fibroblast-like cells (RA-FLS) model. Results PEL could alleviate paw swelling, destruction, rats, significantly superior that EEL WEL. reduced levels, attenuated upregulation rats. Network results indicated myrtenal β-caryophyllene oxide main two PEL, these compounds showed significant TNF-α, RA-FLS. Conclusions Myrtenal screened from suppress inflammasome, thereby alleviating RA symptoms.